Metabolism of T-2 toxin in Curtobacterium sp. strain 114-2

Appl Environ Microbiol. 1983 Jul;46(1):120-7. doi: 10.1128/aem.46.1.120-127.1983.

Abstract

The metabolic pathway of T-2 toxin in Curtobacterium sp. strain 114, one of the T-2 toxin-assimilating soil bacteria, was investigated by thin-layer and gas-liquid chromatographic analyses. T-2 toxin added to the basal medium as a single carbon and energy source was biotransformed into HT-2 toxin and an unknown metabolite. Infrared, mass spectrum, proton magnetic resonance, and other physico-chemical analyses identified this new metabolite as T-2 triol. T-2 toxin was first deacetylated by the bacterium into HT-2 toxin, and this metabolite was then biotransformed into T-2 triol without formation of neosolaniol and T-2 tetraol. No trichothecenes remained in the culture medium after prolonged culture. Some properties of T-2 toxin-hydrolyzing enzymes were observed with whole cells, the cell-free soluble fraction, and the culture filtrate. Besides T-2 toxin, trichothecenes such as diacetoxyscirpenol, neosolaniol, nivalenol, and fusarenon-X were also assimilated by this bacterium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / enzymology
  • Bacteria / metabolism*
  • Biotransformation
  • Kinetics
  • Sesquiterpenes / metabolism*
  • T-2 Toxin / analogs & derivatives
  • T-2 Toxin / metabolism*
  • Trichothecenes / metabolism

Substances

  • Sesquiterpenes
  • Trichothecenes
  • scirpentriol
  • T-2 Toxin
  • HT-2 toxin