A sensitive and specific stable isotope assay for warfarin and its metabolites

Biomed Mass Spectrom. 1983 Jul;10(7):395-8. doi: 10.1002/bms.1200100702.


A capillary gas chromatographic mass spectrometric method for the quantification of warfarin and its known metabolites from microsomal incubations is described. Deuterium labelled 4', 6-, 7- and 8-hydroxy warfarins are used as internal standards and the method has detection limits of 1 ng ml-1 with 20 ng ml-1 being the lower limit for accurate quantification.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biotransformation
  • Gas Chromatography-Mass Spectrometry / methods
  • In Vitro Techniques
  • Kinetics
  • Microsomes, Liver / metabolism
  • Rats
  • Stereoisomerism
  • Warfarin / analysis*


  • Warfarin