In vitro neurotoxic esterase assay using leptophos oxon analogs as inhibitors

Toxicol Lett. 1983 Jun;17(1-2):107-11. doi: 10.1016/0378-4274(83)90044-9.

Abstract

In comparing neurotoxic esterase (NTE) inhibition properties of a series of phenylphosphonates, it was discovered that certain compounds including leptophos inhibited mipafox-insensitive phenylvalerate hydrolases. This leads to erroneous values for NTE inhibition which can be corrected by a differential assay: the total amount of mipafox-insensitive activity is determined with O-(2,6-dichlorophenyl)O-methyl phenylphosphonate and subtracted from the apparent NTE determined with the test compound before calculating pI50's.

MeSH terms

  • Animals
  • Brain / drug effects
  • Brain / enzymology*
  • Carboxylic Ester Hydrolases / antagonists & inhibitors*
  • Chickens
  • Insecticides / toxicity*
  • Leptophos* / analogs & derivatives*
  • Organophosphorus Compounds / toxicity
  • Structure-Activity Relationship

Substances

  • Insecticides
  • Organophosphorus Compounds
  • leptophos oxon
  • Leptophos
  • Carboxylic Ester Hydrolases
  • neurotoxic esterase