A radioimmunoassay for 6-keto-prostaglandin F1alpha

Prostaglandins. 1978 Mar;15(3):383-97. doi: 10.1016/0090-6980(78)90122-3.

Abstract

A radioimmunoassay for 6-keto-prostaglandin F1alpha has been developed. The assay is accurate and sensitive but since the antiserum cross-reacts 5-10% with prostaglandins (PGs) of the E and F series, solvent extraction and thin layer chromatography are required for absolute specificity. The assay has been validated by comparison with a radiochemical assay and by the use of an inhibitor of 6-keto PGF1alpha formation, 15-hydroperoxy arachidonic acid. 6-Keto PGF1alpha was found to have a low cross reaction with antisera directed against PGE2, PGF2alpha and thromboxane B2.

MeSH terms

  • Animals
  • Chromatography, Thin Layer
  • Cross Reactions
  • Immune Sera
  • Microsomes / metabolism
  • Prostaglandins F / analysis*
  • Prostaglandins F / biosynthesis
  • Prostaglandins F / metabolism
  • Prostaglandins H / metabolism
  • Radioimmunoassay / methods*
  • Sheep
  • Thromboxane B2 / analysis

Substances

  • Immune Sera
  • Prostaglandins F
  • Prostaglandins H
  • Thromboxane B2