Antigenicity and evolution amongst recent influenza viruses of H1N1 subtype

Nucleic Acids Res. 1983 Oct 25;11(20):7191-203. doi: 10.1093/nar/11.20.7191.


The sequence of the HA1 subunit region of the haemagglutinin gene of influenza A/USSR/90/77, and A/Brazil/11/78, A/Lackland/3/78, A/England/333/80 and A/India/6263/80 was determined by dideoxy-sequencing methods using total virion RNA and specific oligonucleotide primers for reverse transcriptase. These 1977-1980 strains share a minimum of 85% amino acid sequence homology with influenza A/PR/8/34. Most of the surface amino acid substitutions which occurred during the evolution of A/PR/8/34 to A/USSR/90/77 and subsequently in the 1978-1980 strains are located in the 4 antigenic sites previously defined by an analysis of laboratory-selected mutants of A/PR/8/34. We deduce an evolutionary pathway for the 1977-80 strains and suggest their different epidemic properties may be a consequence of only a few amino acid changes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Viral / genetics*
  • Base Sequence
  • Biological Evolution*
  • Hemagglutination, Viral
  • Hemagglutinins, Viral / genetics*
  • Influenza A Virus, H1N1 Subtype*
  • Influenza A virus / genetics
  • Influenza A virus / immunology*
  • RNA, Viral / genetics
  • Species Specificity
  • Virion / genetics


  • Antigens, Viral
  • Hemagglutinins, Viral
  • RNA, Viral

Associated data

  • GENBANK/X00027
  • GENBANK/X00028
  • GENBANK/X00029
  • GENBANK/X00030
  • GENBANK/X00031