The studies were carried out on 15 rabbits in three experimental series, divided into three groups in relation to the diet: group 0 - on a standard diet, group I - a standard diet with cholesterol added, group II - a standard diet with cholesterol and vitamin B15. After decapitating the rabbits, the level of total, free and bound cholesterol was determined by the method of Liberman and Burchart in the wall of the stomach, jejunum, duodenum, caecum, colon and liver. It was shown that vitamin B15 caused a decrease of cholesterol level in the wall of the stomach, intestines and liver of the rabbits. It was simultaneously observed that cholesterol content in the particular intestine segments was different - high in caecum, but low in jejunum. In rabbits on a diet rich in cholesterol, an increase of its level was observed in the walls of the alimentary canal.