A simple spectrophotometric method for the direct determination of uric acid in avian excreta

Poult Sci. 1983 Oct;62(10):2106-8. doi: 10.3382/ps.0622106.

Abstract

A procedure was developed for the direct quantitation of uric acid in poultry excreta. It involved the determination of the absorbance of a perchloric acid extract of excreta at 285 nm. Interference from other ultraviolet absorbing material was shown to be negligible. The correlation (r) between this procedure and a recently developed high-performance liquid chromatographic procedure for uric acid content of excreta from birds fed different diets was .993 (P less than .01). It was concluded that this procedure provides a simple and reliable method for the quantitation of uric acid in avian excreta.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens
  • Chromatography, High Pressure Liquid
  • Feces / analysis*
  • Female
  • Hydrogen-Ion Concentration
  • Male
  • Spectrophotometry, Ultraviolet / methods
  • Uric Acid / analysis*

Substances

  • Uric Acid