Radioimmunoassay of 2-hydroxyestrone

Steroids. 1978 Feb;31(2):249-58. doi: 10.1016/0039-128x(78)90117-4.


Under the protection of ascorbic acid a 2-hydroxyestrone bovine serum albumin conjugate was prepared containing intact 2-hydroxyestrone as determined by gas chromatographymass spectometry. Using this antigen highely specific antibodies were raised in rabbits. Cross-reactivity for 2-hydroxyestradiol and 2-hydroxyestriol was 26 and 4.5%, respectively. An assay procedure of 2-hydroxyestrone in human plasma is described. Using special precautions the assay allows the determination of 2-hydroxyestrone in plasma samples of women (50-95 pg/ml), pregnant women (105-220 pg/ml), men (45-65 pg/ml) and children(20-40 pg/ml).

MeSH terms

  • Adult
  • Antigen-Antibody Reactions
  • Child
  • Child, Preschool
  • Cross Reactions
  • Estrone / analogs & derivatives*
  • Female
  • Humans
  • Hydroxyestrones / blood*
  • Kinetics
  • Male
  • Middle Aged
  • Pregnancy
  • Radioimmunoassay / methods


  • Hydroxyestrones
  • Estrone