6 beta-hydroxycortisol in random urine samples as an indicator of enzyme induction

Clin Pharmacol Ther. 1983 Dec;34(6):818-21. doi: 10.1038/clpt.1983.255.


6 beta-Hydroxycortisol (6 beta-OHF) is a major cortisol metabolite formed primarily in the hepatic reticulum by mixed-function oxygenases. Changes in 6 beta-OHF may be used to detect enzyme induction by drugs or disease. Excretion of 6 beta-OHF must be corrected for change in urinary free cortisol (FF) excretion to allow for changes in cortisol production. We report the absence of circadian changes in 6 beta-OHF/FF ratios. Thus random measurements of 6 beta-OHF/FF ratios may be used to detect enzyme induction. This approach greatly simplifies noninvasive screening for enzyme induction.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Child
  • Child, Preschool
  • Circadian Rhythm
  • Enzyme Induction / drug effects
  • Female
  • Humans
  • Hydrocortisone / analogs & derivatives*
  • Hydrocortisone / urine
  • Male
  • Mixed Function Oxygenases / biosynthesis*
  • Phenobarbital / pharmacology


  • 6 beta-hydroxycortisol
  • Mixed Function Oxygenases
  • Hydrocortisone
  • Phenobarbital