Diazepam inhibits cell respiration and induces fragmentation of mitochondrial reticulum

FEBS Lett. 1983 Nov 14;163(2):311-4. doi: 10.1016/0014-5793(83)80842-4.


Diazepam (70-150 micrograms/ml) significantly inhibits oxygen consumption by pig kidney embryo cells and causes the cellular ATP level to fall. The maximum inhibitory effect develops after 1.5-2.5 h of diazepam treatment. In isolated mitochondria diazepam inhibits respiration in state 2 and 3u with glutamate and in state 3u with succinate. Ethylrhodamine staining and electron microscopic study reveal fragmentation of mitochondria in living cells.

MeSH terms

  • Animals
  • Cells, Cultured
  • Diazepam / pharmacology*
  • Fluorescent Dyes
  • Kidney
  • Mitochondria / drug effects
  • Mitochondria / metabolism*
  • Mitochondria / ultrastructure
  • Oxygen Consumption / drug effects*
  • Rhodamines
  • Swine


  • Fluorescent Dyes
  • Rhodamines
  • Diazepam