Dot-blotting--a novel screening assay for antibodies in hybridoma cultures

J Immunol Methods. 1983 Nov 11;64(1-2):39-43. doi: 10.1016/0022-1759(83)90382-4.

Abstract

A novel solid phase radioimmunoassay using nitrocellulose filters, is described. The method is of particular value in screening hybridoma cultures for monoclonal antibodies against chromosomal protein. Also it permits detection of antibodies directed against epitopes stable to SDS denaturation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / analysis*
  • Antigen-Antibody Complex
  • Antigens / analysis
  • Chromosomes / analysis*
  • Collodion
  • Cricetinae
  • Cricetulus
  • Hybridomas / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Nucleoproteins / analysis*
  • Paper
  • Radioimmunoassay / methods

Substances

  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Antigens
  • Nucleoproteins
  • Collodion