Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development

Nature. 1983 Dec 8-14;306(5943):551-7. doi: 10.1038/306551a0.


Nucleotide sequence and deletion analysis have been used to identify the regulatory and coding sequences comprising the cholera toxin operon (ctx). Incorporation of defined in vitro-generated ctx deletion mutations into Vibrio cholerae by in vivo genetic recombination produced strains which have practical value in cholera vaccine development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cholera Toxin / genetics*
  • Cholera Vaccines*
  • Cloning, Molecular
  • Gene Expression Regulation
  • Operon
  • Recombination, Genetic*
  • Vibrio cholerae / genetics


  • Cholera Vaccines
  • Cholera Toxin

Associated data

  • GENBANK/X00171