Chloride activity in cells of isolated perfused cortical thick ascending limbs of rabbit kidney

Pflugers Arch. 1983 Sep;399(1):29-34. doi: 10.1007/BF00652518.

Abstract

Rabbit cortical thick ascending limb segments were perfused in vitro, and intracellular Cl- activity was estimated in three types of experiments using conventional and chloride selective microelectrodes. In series 1 Ringer like solutions were present on the two epithelial sides. In series 2 limen Cl- was replaced by gluconate, and in series 3 furosemide, 10-20 . 10(-6) mol . 1-1, was added to the lumen perfusate. It was found that under control conditions intracellular Cl- activity, as estimated from the difference of the reading of the conventional (n = 53) and ion selective electrodes (n = 118) was 26 +/- 1 mmol . 1-1. Thi value is approximately three times higher than expected for passive distribution of Cl-. After removal of lumen Cl- (series 2) intracellular Cl- activity fell to 9 mmol . 1(-1) which is only some 4 mmol . 1(-1) above passive distribution. We argue that these 4 mmol . 1(-1) reflect mainly the interference with the Cl- electrode by other anions, such as phosphate. The above estimates for intracellular Cl- activity, have to be diminished by these 4 mmol . 1(-1), and, thus, are close to 22 mmol . 1(-1). In series 3 a rapid and reversible fall in intracellular Cl- from 23 to 7 mmol . 1(-1) was observed. We conclude that the Cl- activity in cTAL cells is clearly above equilibrium under control conditions and that it falls rapidly to values close to equilibrium when Cl- reabsorption is blocked by either removing lumen Cl- or by blocking the Cl- entry via the Na+-2 Cl--K+-carrier with furosemide.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / pharmacology
  • Chlorides / pharmacology
  • Chlorine / metabolism*
  • Culture Techniques
  • Female
  • Furosemide / pharmacology
  • Gluconates / pharmacology
  • Glucose / pharmacology
  • Kidney Tubules / cytology*
  • Loop of Henle / cytology*
  • Loop of Henle / metabolism
  • Perfusion
  • Phosphoric Acids / pharmacology
  • Potassium / pharmacology
  • Rabbits
  • Sodium / pharmacology
  • Solutions

Substances

  • Chlorides
  • Gluconates
  • Phosphoric Acids
  • Solutions
  • Chlorine
  • Furosemide
  • Sodium
  • Glucose
  • Potassium
  • Calcium