Preparation and Use of Nuclease-Treated Rabbit Reticulocyte Lysates for the Translation of Eukaryotic Messenger RNA

Methods Enzymol. 1983;96:50-74. doi: 10.1016/s0076-6879(83)96008-1.

MeSH terms

  • Animals
  • Cell Separation
  • Cell-Free System
  • Electrophoresis, Polyacrylamide Gel / methods
  • Eukaryotic Cells / metabolism
  • Female
  • Indicators and Reagents
  • Male
  • Micrococcal Nuclease
  • Protein Biosynthesis*
  • Proteins / genetics
  • Proteins / isolation & purification
  • RNA, Messenger / genetics*
  • Rabbits
  • Reticulocytes / cytology
  • Reticulocytes / metabolism*

Substances

  • Indicators and Reagents
  • Proteins
  • RNA, Messenger
  • Micrococcal Nuclease