Immunohistochemical demonstration of beta-enolase in human skeletal muscle

Muscle Nerve. Nov-Dec 1983;6(9):661-3. doi: 10.1002/mus.880060907.

Abstract

We have localized beta-enolase activity in human skeletal muscle fiber using the immunohistochemical method (two-step method). The first immunoreagent was rabbit anti-human beta-enolase serum raised in New Zealand white rabbit, and the second was peroxidase conjugated staphylococcal protein A. The immunohistochemical reaction for beta-enolase was noted higher in type 2 fiber, which demonstrates the low oxidative and high glycolytic enzyme activity, than in type 1 fiber.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Histocytochemistry / methods
  • Humans
  • Immunochemistry / methods
  • Muscles / enzymology*
  • Phosphopyruvate Hydratase / metabolism*

Substances

  • Phosphopyruvate Hydratase