An improved colony hybridization method with significantly increased sensitivity for detection of single genes

Plasmid. 1983 Nov;10(3):296-8. doi: 10.1016/0147-619x(83)90045-8.

Abstract

By introducing a simple modification of existing methods, colony hybridization has been used to detect single copy genes coding for a heat-stable enterotoxin in wild-type strains isolated from patients with diarrhea. The modification described in this communication results in an approximately 100-fold increase in sensitivity, probably by increasing the total denatured plasmid DNA fixed to the paper.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cloning, Molecular / methods*
  • Hot Temperature
  • Nucleic Acid Hybridization*
  • Time Factors