Purified DNA polymerase gamma replicates bovine parvovirus DNA to a unit-length product

Biochem Biophys Res Commun. 1983 Dec 16;117(2):580-6. doi: 10.1016/0006-291x(83)91240-8.


DNA polymerase gamma, purified from fetal bovine liver, replicated virion single-stranded DNA from bovine parvovirus to a unit-length double-stranded DNA molecule. This product was not nicked and was covalently linked to the 3' hairpin primer. The reaction was inhibited by dideoxythymidine 5'-triphosphate, but was unaffected by ATP or aphidicolin. Double-stranded viral DNA was not a functional template for purified DNA polymerase gamma.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenosine Triphosphate / pharmacology
  • Animals
  • Cattle
  • DNA Polymerase III / isolation & purification
  • DNA Polymerase III / metabolism*
  • DNA Replication*
  • DNA, Viral / biosynthesis*
  • DNA-Directed DNA Polymerase / metabolism*
  • Female
  • Liver / enzymology
  • Parvoviridae / genetics*
  • Pregnancy


  • DNA, Viral
  • Adenosine Triphosphate
  • DNA Polymerase III
  • DNA-Directed DNA Polymerase