Abstract
DNA polymerase gamma, purified from fetal bovine liver, replicated virion single-stranded DNA from bovine parvovirus to a unit-length double-stranded DNA molecule. This product was not nicked and was covalently linked to the 3' hairpin primer. The reaction was inhibited by dideoxythymidine 5'-triphosphate, but was unaffected by ATP or aphidicolin. Double-stranded viral DNA was not a functional template for purified DNA polymerase gamma.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Adenosine Triphosphate / pharmacology
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Animals
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Cattle
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DNA Polymerase III / isolation & purification
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DNA Polymerase III / metabolism*
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DNA Replication*
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DNA, Viral / biosynthesis*
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DNA-Directed DNA Polymerase / metabolism*
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Female
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Liver / enzymology
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Parvoviridae / genetics*
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Pregnancy
Substances
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DNA, Viral
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Adenosine Triphosphate
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DNA Polymerase III
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DNA-Directed DNA Polymerase