Structural organization of unique retrovirus-like particles budding from human teratocarcinoma cell lines

J Gen Virol. 1983 Dec;64 ( Pt 12):2549-59. doi: 10.1099/0022-1317-64-12-2549.


Human teratocarcinoma cells cultured in vitro can be induced to produce retrovirus-like particles. The induction procedures are the same as those previously shown to induce the synthesis of animal retroviruses. Electron microscopical evidence is presented that the human teratocarcinoma-derived (HTD) particles are most closely related to the type C retrovirus strains. HTD particles can be banded at 1.16 g/ml in linear sucrose gradients, the characteristic density for retroviruses, and subsequently be used for negative staining and fine structure analysis.

Publication types

  • Comparative Study

MeSH terms

  • Cell Line
  • Centrifugation, Density Gradient
  • Humans
  • Microscopy, Electron
  • Retroviridae / growth & development
  • Retroviridae / ultrastructure*
  • Teratoma / microbiology*