Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 524 (2), 254-61

Rapid Affinity Purification and Properties of Rat Liver Sorbitol Dehydrogenase

Rapid Affinity Purification and Properties of Rat Liver Sorbitol Dehydrogenase

N Leissing et al. Biochim Biophys Acta.

Abstract

A 23-h affinity chromatography purification procedure for sorbitol dehydrogenase (L-iditol:NADl-oxidoreductase, EC 1.1.1.14) prepared from freshly excised rat liver has been developed that resulted in an 18% yield of an apparently homogeneous preparation (purification = 439-fold). The molecular weight of the enzyme was approx. 96 000. The enzyme was specific for NAD+ (NADH), but had no requirement for NADP+ (NADPH). The purified preparation shows significant activity with structurally related polyols and ketoses. Km values for sorbitol and fructose are 0.35 and 110 mM (at pH 7.1), respectively.

Similar articles

See all similar articles

Cited by 3 PubMed Central articles

Substances

LinkOut - more resources

Feedback