Evidence of 2-hydroxylation of estradiol-17 beta 17-glucuronide by male rat liver microsomes

Steroids. 1983 Aug;42(2):163-70. doi: 10.1016/s0039-128x(83)90184-8.


When estradiol-17 beta 17-glucuronide was incubated with male rat liver microsomal preparations with a NADPH-generating system, 2-hydroxyestradiol-17 beta 17-glucuronide was obtained. This 2-hydroxylation was shown to occur without cleavage of the conjugate group. The result clearly indicates that estradiol-17 beta 17-glucuronide could act as substrate for rat liver microsomal 2-hydroxylase.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cytochrome P-450 CYP1A1*
  • Estradiol / analogs & derivatives*
  • Estradiol / metabolism
  • Hydroxylation
  • In Vitro Techniques
  • Male
  • Microsomes, Liver / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Steroid Hydroxylases / metabolism


  • estradiol-17 beta-glucuronide
  • Estradiol
  • Steroid Hydroxylases
  • Cytochrome P-450 CYP1A1
  • estrogen 2-hydroxylase