Increased Purine Nucleotide Binding, Altered Polypeptide Composition, and Thermogenesis in Brown Adipose Tissue Mitochondria of Cold-Acclimated Rats

Can J Biochem. 1978 Jun;56(6):378-83. doi: 10.1139/o78-060.

Abstract

Rapid increases in atractyloside-insensitive binding of purine nucleotides (ADP or GDP) and in a polypeptide of 32 000 occur in brown adipose tissue mitochondria of the rat during acclimation to cold. The increased binding is apparent within 1 h and reaches a maximum after 3--7 days of exposure to 4 degrees C. The increase in the 32 000 peptide occurs more slowly and reaches a maximum after 2--3 weeks. There is a simultaneous decrease in a polypeptide of 96 000, apparent after 1 day and reaching a maximum after 1--2 weeks. Results are interpreted in terms of the appearance of an increased amount of the purine nucleotide-sensitive proton conductance pathway in association with the development of an enhanced thermogenic capacity of brown adipose tissue mitochondria during acclimation of the rat to cold.

MeSH terms

  • Acclimatization*
  • Adenosine Diphosphate / metabolism
  • Adipose Tissue, Brown / metabolism*
  • Animals
  • Cold Temperature*
  • Energy Metabolism
  • Guanosine Diphosphate / metabolism
  • Male
  • Mitochondria / metabolism
  • Molecular Weight
  • Peptides / metabolism
  • Protein Binding
  • Purine Nucleotides / metabolism*
  • Rats

Substances

  • Peptides
  • Purine Nucleotides
  • Guanosine Diphosphate
  • Adenosine Diphosphate