The purpose of this study was to measure the VO2max of trained cyclists on the treadmill (means +/- SD = 54.7 +/- 6.3 ml kg-1 min-1), while riding a bicycle on a velodrome track at 100 rpm (53.7 +/- 7.8) and on the bicycle ergometer at 60 rpm (62.4 +/- 8.1): VO2max beeing the highest in the latter case (p less than 0.05). The highest maximal HR, 188 +/- 6 beats min-1, was observed during the treadmill test, while estimates of 184 +/- 6 and 179 +/- 7 were obtained for the velodrome and the bicycle ergometer tests, respectively. No significant differences were observed in the blood lactate concentrations (treadmill: 10.35 +/- 4.01 bicycle ergometer: 10.25 +/- 2.29 velodrome: 10.95 +/- 1.51 mmol L-1. In conclusion, bicycle ergometer tests might not be specific enough to evaluate the ability of trained cyclists to perform an endurance or aerobic task on the track. Trained cyclists, as opposed to untrained ones, appear to achieve higher VO2max on the bicycle ergometer as compared to the treadmill.