Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX

Nucleic Acids Res. 1983 Apr 25;11(8):2325-35. doi: 10.1093/nar/11.8.2325.

Abstract

A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisely one codon has been deleted.Images

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Cloning, Molecular*
  • Codon / genetics
  • DNA / isolation & purification*
  • Factor IX / genetics*
  • Hemophilia A / genetics*
  • Humans
  • Liver / metabolism
  • Oligodeoxyribonucleotides*
  • Oligonucleotides*
  • Species Specificity

Substances

  • Codon
  • Oligodeoxyribonucleotides
  • Oligonucleotides
  • Factor IX
  • DNA
  • Alanine

Associated data

  • GENBANK/J00137
  • GENBANK/J02523