A Simple and Rapid Enzymatic Determination of L-phenylalanine With a Novel L-phenylalanine Oxidase (Deaminating and Decarboxylating) From Pseudomonas Sp. P-501

Clin Chim Acta. 1984 Jan 31;136(2-3):131-6. doi: 10.1016/0009-8981(84)90284-5.

Abstract

A simple and rapid method for the determination of L-phenylalanine using Pseudomonas L-phenylalanine oxidase (deaminating and decarboxylating) has been developed. This method involves the oxidative deamination of L-phenylalanine using L-phenylalanine oxidase from Pseudomonas sp. P-501, and the colorimetric determination of hydrogen peroxide formed from L-phenylalanine with 4-aminoantipyrine and N,N'-dimethylaniline in the presence of peroxidase. This method requires a smaller quantity of sample and is less time-consuming than those previously reported. The method can be used for the direct assay of serum L-phenylalanine levels without pretreatment.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Oxidoreductases / metabolism*
  • Autoanalysis
  • Humans
  • Phenylalanine / analysis*
  • Phenylalanine / blood
  • Pseudomonas / enzymology*
  • Spectrophotometry

Substances

  • Phenylalanine
  • Amino Acid Oxidoreductases
  • phenylalanine oxidase