An efficient method for labelling antibodies with 111In

FEBS Lett. 1984 Jan 23;166(1):202-4. doi: 10.1016/0014-5793(84)80073-3.

Abstract

Using a new method, rabbit IgG and a monoclonal antibody have been conjugated with the chelating agent DTPA. This was accomplished with reaction conditions that should entail lower antibody damage than existing methods. Gel filtration of the 111In-labelled antibody conjugate indicated minimal damage to the antibody and radioimmunoassay showed no significant change in its immunological activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies
  • Chelating Agents
  • Chemical Phenomena
  • Chemistry
  • Indium*
  • Pentetic Acid
  • Radioimmunoassay / methods*
  • Radioisotopes*

Substances

  • Antibodies
  • Chelating Agents
  • Radioisotopes
  • Indium
  • Pentetic Acid