Effect of chromosomal position on amplification of transfected genes in animal cells

Nature. 1984 Feb 9-15;307(5951):516-20. doi: 10.1038/307516a0.


A single protein (CAD) contains the first three enzymatic activities of de novo uridine biosynthesis. The chromosomal location of CAD genes introduced into Chinese hamster ovary cells significantly affects the frequency and cytogenetic result of their amplification. The amplification frequency in one transformant is 100-fold that of the others; in another, amplification of donated genes inserted near a centromere results in chromosome instability and rearrangements.

MeSH terms

  • Animals
  • Aspartate Carbamoyltransferase*
  • Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)*
  • Cells, Cultured
  • Chromosome Mapping
  • Cricetinae
  • Dihydroorotase*
  • Gene Amplification*
  • Gene Expression Regulation*
  • Genetic Linkage
  • Multienzyme Complexes*
  • Proteins / genetics
  • Transfection


  • CAD trifunctional enzyme
  • Multienzyme Complexes
  • Proteins
  • Aspartate Carbamoyltransferase
  • Dihydroorotase
  • Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)