Selection of therapeutic agents for intraocular proliferative disease. Cell culture evaluation

Arch Ophthalmol. 1984 Apr;102(4):598-604. doi: 10.1001/archopht.1984.01040030470029.


A variety of antimetabolites, steroids, and nonsteroidal anti-inflammatory agents were tested for their ability to inhibit rabbit dermal and conjunctival fibroblast proliferation in cell culture. Doxorubicin hydrochloride and fluorouracil produced notable inhibition in concentrations of less than 1 mg/L. Meclofenamate sodium and indomethacin produced notable inhibition at concentrations of 11 and 40 mg/L, respectively. Dexamethasone sodium phosphate and triamcinolone acetonide produced inhibition at 200 and 150 mg/L, respectively, but paradoxically increased proliferation almost two-fold at concentrations ranging from 1 to 30 mg/L under identical culture conditions. Methotrexate sodium demonstrated only limited effectiveness. This assay system may be a useful approach to drug selection in the treatment of a variety of ocular proliferative disorders. Fluorouracil may prove to be of significant value in the treatment of intraocular proliferative disorders.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Antimetabolites / pharmacology*
  • Cell Division
  • Cells, Cultured
  • Dexamethasone / pharmacology
  • Doxorubicin / pharmacology
  • Eye / drug effects*
  • Eye Diseases / drug therapy
  • Fibroblasts / drug effects*
  • Fibroblasts / physiology
  • Indomethacin / pharmacology
  • Meclofenamic Acid / pharmacology
  • Methotrexate / pharmacology
  • Rabbits
  • Triamcinolone / pharmacology


  • Anti-Inflammatory Agents
  • Antimetabolites
  • Triamcinolone
  • Meclofenamic Acid
  • Dexamethasone
  • Doxorubicin
  • Indomethacin
  • Methotrexate