Abstract
Intracellular free Ca+2 concentration was measured in cultured human fibroblasts (HSWP) utilizing the Ca+2-sensitive fluorescent probe quin2. The addition of peptide growth factors to serum-deprived HSWP cells induced an immediate rise in intracellular Ca+2 concentration. This mitogen-induced rise in Ca+2 concentration could be blocked by the addition of the intracellular Ca+2 antagonist TMB-8. Addition of the phospholipase activator, melittin, to cells in the absence of growth factors also caused a dramatic rise in intracellular Ca+2 concentration which was blocked by TMB-8.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aminoquinolines
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Bee Venoms / pharmacology*
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Calcium / metabolism*
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Calcium Channel Blockers / pharmacology
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Cells, Cultured
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Cytoplasm / metabolism
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Fibroblasts / drug effects
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Fibroblasts / metabolism*
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Fluorescent Dyes
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Gallic Acid / analogs & derivatives
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Gallic Acid / pharmacology
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Humans
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Male
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Melitten / pharmacology*
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Mitogens / pharmacology*
Substances
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Aminoquinolines
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Bee Venoms
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Calcium Channel Blockers
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Fluorescent Dyes
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Mitogens
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Melitten
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8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate
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Gallic Acid
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Quin2-acetoxymethyl ester
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Calcium