Iodide transport in a continuous line of cultured cells from rat thyroid

Endocrinology. 1984 Apr;114(4):1090-8. doi: 10.1210/endo-114-4-1090.


The properties of TSH-dependent iodide (I-) uptake are defined for a cloned, continuously growing, functioning rat thyroid cell line (FRTL-5 cells). Since these cells grow without a lumen and are therefore restricted in their ability to iodinate thyroglobulin, the FRTL-5 cells offer the opportunity to directly study transport processes across the membrane into the cell as well as the process whereby I- moves from the cell. FRTL-5 cells concentrate I- approximately 30-fold and exhibit many of the properties of I- uptake seen in thyroid tissue slice and primary cell culture systems. In these cells, accumulation of I- is consistent with a Na+-dependent carrier model for I- uptake, and effects on the influx and efflux processes can be dissociated. Because FRTL-5 cells can be maintained in culture indefinitely and can provide large quantities of a homogeneous functional thyroid cell preparation for study, these cells offer the unique opportunity to further define the mechanism and kinetics of I- transport in a less complex system.

MeSH terms

  • 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
  • 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / analogs & derivatives
  • 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / pharmacology
  • Aminoisobutyric Acids / metabolism
  • Animals
  • Biological Transport / drug effects
  • Carbon Radioisotopes
  • Cell Line
  • Hydrogen-Ion Concentration
  • Iodides / metabolism*
  • Iodine Radioisotopes
  • Kinetics
  • Potassium / pharmacology
  • Rats
  • Temperature
  • Thyroid Gland / metabolism*
  • Thyrotropin / pharmacology*


  • Aminoisobutyric Acids
  • Carbon Radioisotopes
  • Iodides
  • Iodine Radioisotopes
  • 2-aminoisobutyric acid
  • 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
  • Thyrotropin
  • 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
  • Potassium