Comparison of 1-acylglycerophosphate and Glycerophosphate Acyltransferases From Euglena Microsomes

Int J Biochem. 1984;16(2):219-23. doi: 10.1016/0020-711x(84)90076-4.

Abstract

Both glycerophosphate and monoacylglycerophosphate acyltransferases from Euglena microsomes were inhibited by N-ethylmaleimide, but their responses to heat inactivation and sn-glyceraldehyde-3-phosphate differed. Glycerophosphate acyltransferase had a higher V with palmitoyl-CoA compared to oleoyl-CoA; the reverse was true for monoacylglycerophosphate acyltransferase. Km's (microM) for the glycerophosphate acyltransferase were: palmitoyl-CoA, 21; oleoyl-CoA, 14; and sn-glycerol-3-phosphate, 2900. Km's (microM) for monoacylglycerophosphate acyltransferase were: palmitoyl-CoA, 7; oleoyl-CoA, 4; and 1-palmitoyl-sn-glycerol-3-phosphate, 48.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 1-Acylglycerol-3-Phosphate O-Acyltransferase
  • Acyltransferases / antagonists & inhibitors
  • Acyltransferases / isolation & purification*
  • Catalysis
  • Euglena / enzymology*
  • Glycerol-3-Phosphate O-Acyltransferase / antagonists & inhibitors
  • Glycerol-3-Phosphate O-Acyltransferase / isolation & purification*
  • Hot Temperature
  • Microsomes / enzymology*
  • Substrate Specificity

Substances

  • Acyltransferases
  • Glycerol-3-Phosphate O-Acyltransferase
  • 1-Acylglycerol-3-Phosphate O-Acyltransferase