Rat liver histidyl-tRNA synthetase. Purification and inhibition by the myositis-specific anti-Jo-1 autoantibody

Biochem Biophys Res Commun. 1984 Apr 16;120(1):15-21. doi: 10.1016/0006-291x(84)91407-4.


Myositis is an autoimmune inflammatory muscle disease of unknown etiology. We demonstrate directly that the antigen to the myositis-specific anti-Jo-1 antibody is histidyl-tRNA synthetase. The anti-Jo-1 antibody inhibits human HeLa and rat liver histidyl-tRNA synthetase. Using conventional and immunoaffinity chromatography with immobilized anti-Jo-1 antibody, we have purified rat liver histidyl-tRNA synthetase which has a subunit Mr 64,000 and an estimated native Mr suggesting an alpha 2 structure. The evidence indicates that the Jo-1 antigen is histidyl-tRNA synthetase, and that some of the histidyl-tRNA synthetase structure are conserved across species.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acyl-tRNA Synthetases / isolation & purification*
  • Animals
  • Autoantibodies / pharmacology*
  • Chromatography, Affinity
  • HeLa Cells
  • Histidine-tRNA Ligase / antagonists & inhibitors
  • Histidine-tRNA Ligase / isolation & purification*
  • Humans
  • Immunochemistry
  • Liver / enzymology*
  • Myositis / immunology*
  • Rats


  • Autoantibodies
  • Amino Acyl-tRNA Synthetases
  • Histidine-tRNA Ligase