Monoclonal antibody ECCD-1 inhibits intercellular communication in teratocarcinoma PCC3 cells

Exp Cell Res. 1984 May;152(1):270-4. doi: 10.1016/0014-4827(84)90253-2.

Abstract

The monoclonal antibody ECCD-1 recognizing a certain class of cell surface proteins inhibits the Ca2+-dependent cell-to-cell adhesion in teratocarcinoma stem cells. In this paper, we studied the effect of ECCD-1 on cell-to-cell communication in PCC3 cells by measuring the transfer of lucifer yellow between cells. To this aim, PCC3 cells were cultured in the presence of ECCD-1 for various periods, and then the fluorescent dye was injected into a cell located in the center of cell colonies, followed by counting number of cells to which the dye was transferred. The results showed that ECCD-1 inhibits the dye transfer between cells, suggesting that the Ca2+-dependent cell-to-cell adhesion system (CDS) is essential for the functions of gap junction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Cell Adhesion*
  • Cell Communication*
  • Cell Line
  • Isoquinolines / metabolism
  • Membrane Proteins / immunology
  • Membrane Proteins / physiology*
  • Neoplasm Proteins / immunology
  • Neoplasm Proteins / physiology*
  • Teratoma / physiopathology*

Substances

  • Antibodies, Monoclonal
  • Isoquinolines
  • Membrane Proteins
  • Neoplasm Proteins
  • lucifer yellow