Assay of intestinal disaccharidases

Scand J Clin Lab Invest. 1984 Apr;44(2):169-72. doi: 10.3109/00365518409161400.


A modified and updated description of the author's method for the assay of intestinal disaccharidases. The principle of the method is the following: an intestinal homogenate is incubated with the appropriate disaccharide. The disaccharidase activity is then interrupted by the addition of TRIS, and the glucose liberated is measured with a glucose oxidase reagent.

MeSH terms

  • Biopsy
  • Disaccharides / analysis*
  • Humans
  • Intestinal Mucosa / analysis*
  • Intestinal Mucosa / pathology
  • Methods
  • Reference Values


  • Disaccharides