Determination of tyrosine exposure in proteins by second-derivative spectroscopy

Biochemistry. 1984 Apr 10;23(8):1871-5. doi: 10.1021/bi00303a044.


The mutual interference between the second-derivative bands of tyrosine and tryptophan in proteins has been evaluated in terms of the ratio r between two peak to peak distances. The r values have been found to be not only related to the tyrosine/tryptophan ratio but also dependent on the polarity of the medium in which tyrosyl residues are embedded. The results obtained on purified proteins have been found consistent with the available X-ray information and with the existing solvent perturbation data.

MeSH terms

  • Acetylation
  • Animals
  • Protein Conformation
  • Proteins*
  • Spectrophotometry, Ultraviolet
  • Tryptophan / analysis
  • Tyrosine / analysis*


  • Proteins
  • Tyrosine
  • Tryptophan