Sequences in the promoter region of the chicken lysozyme gene required for steroid regulation and receptor binding

Cell. 1984 Jun;37(2):503-10. doi: 10.1016/0092-8674(84)90380-5.


We have constructed a series of deletion mutants in the lysozyme promoter region fused to the SV40 T-antigen coding region. Regulated expression was tested after microinjection of the lysozyme deletion mutants into primary cultures of chicken oviduct cells using fluorescent antibodies against T antigen. Deletion of lysozyme gene sequences upstream of position - 164 was accompanied by loss of both progesterone- and glucocorticoid-induced expression. Using the rat liver glucocorticoid receptor for binding studies, two separate binding sites have been identified: a strong binding site that is destroyed by deletion of lysozyme sequences between positions -74 and -39 and a weaker binding site contained between positions -208 and -161 upstream of the lysozyme cap site.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chickens
  • Chromosome Deletion
  • Female
  • Genes*
  • HeLa Cells / metabolism
  • Liver / metabolism
  • Muramidase / genetics*
  • Mutation
  • Operon*
  • Oviducts / metabolism
  • Plasmids
  • Rats
  • Receptors, Glucocorticoid / metabolism*
  • Receptors, Steroid / metabolism*
  • Transfection


  • Receptors, Glucocorticoid
  • Receptors, Steroid
  • Muramidase

Associated data

  • GENBANK/M12532