A Method for the Quantitative Recovery of Protein in Dilute Solution in the Presence of Detergents and Lipids

Anal Biochem. 1984 Apr;138(1):141-3. doi: 10.1016/0003-2697(84)90782-6.

Abstract

A rapid method based on a defined methanol-chloroform-water mixture for the quantitative precipitation of soluble as well as hydrophobic proteins from dilute solutions (e.g., column chromatography effluents) has been developed. The effectiveness of this method is not affected by the presence of detergents, lipids, salt, buffers, and beta-mercaptoethanol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Chemical Precipitation
  • Detergents
  • Electrophoresis, Polyacrylamide Gel
  • Lipids
  • Osmolar Concentration
  • Proteins / analysis*
  • Rabbits
  • Rats
  • Solutions

Substances

  • Detergents
  • Lipids
  • Proteins
  • Solutions