Formation of 19-nor-10-keto-25-hydroxyvitamin D3 in cultured mammalian cells

Biochem Biophys Res Commun. 1984 May 16;120(3):919-25. doi: 10.1016/s0006-291x(84)80194-1.


Cultured dog kidney cells convert 25-hydroxyvitamin D3 into more polar metabolites during in vitro incubations. One elutes with 1,25-dihydroxyvitamin D3 from high pressure liquid chromatographic silica columns with hexane:isopropanol (9:1), but can be separated from 1,25-dihydroxyvitamin D3 by elution with dichloromethane:isopropanol (95:5). This peak has been isolated, purified and identified by mass spectral analysis to be 19-nor-10-keto-25-hydroxyvitamin D3.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcifediol / analogs & derivatives*
  • Calcifediol / biosynthesis
  • Calcifediol / metabolism
  • Cell Line
  • Chromatography, Thin Layer
  • Dogs
  • Kidney
  • Mass Spectrometry


  • 19-nor-10-keto-25-hydroxyvitamin D3
  • Calcifediol