Autocrine regulation of growth: II. Glucocorticoids inhibit transcription of c-sis oncogene-specific RNA transcripts

Biochem Biophys Res Commun. 1984 Jul 18;122(1):124-8. doi: 10.1016/0006-291x(84)90448-0.

Abstract

The ductus deferens smooth muscle tumor cell line (DDT1MF-2) expresses c-sis proto-oncogene poly A+ RNA transcripts which are thought to encode at least one subunit of the potent mitogen platelet derived growth factor (PDGF). We have previously demonstrated that glucocorticoids block DDT1MF-2 cells in G0/G1 stage of the cell cycle, and that exogenously applied PDGF reinitiates cell cycle progression. In this paper we document that glucocorticoids act to inhibit cell cycle progression by inhibiting the expression of c-sis poly A+ transcripts, which we suggest are encoding a PDGF-like molecule for DDT1MF-2 cells.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Cycle
  • Cell Line
  • Gene Expression Regulation*
  • Glucocorticoids / physiology*
  • Muscle, Smooth
  • Neoplasms, Muscle Tissue
  • Oncogenes*
  • Platelet-Derived Growth Factor / physiology*
  • RNA, Messenger / metabolism
  • Transcription, Genetic*

Substances

  • Glucocorticoids
  • Platelet-Derived Growth Factor
  • RNA, Messenger