Chemical modification of Cys-374 of actin interferes with the formation of the profilactin complex

FEBS Lett. 1984 Aug 6;173(2):399-402. doi: 10.1016/0014-5793(84)80813-3.

Abstract

Chemical modification of the cysteine residue 374 of actin, both with N-ethylmaleimide and with the fluorescent probe N-(1-pyrenyl)iodoacetamide, is shown to counteract the inhibiting effect of profilin on actin polymerization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism*
  • Animals
  • Cattle
  • Contractile Proteins / metabolism
  • Cysteine*
  • Ethylmaleimide / pharmacology*
  • Fluorescent Dyes / pharmacology*
  • Iodoacetamide / analogs & derivatives*
  • Iodoacetamide / pharmacology
  • Iodoacetates*
  • Macromolecular Substances
  • Microfilament Proteins*
  • Profilins
  • Proteins / metabolism*
  • Spectrometry, Fluorescence
  • Spleen / metabolism

Substances

  • Actins
  • Contractile Proteins
  • Fluorescent Dyes
  • Iodoacetates
  • Macromolecular Substances
  • Microfilament Proteins
  • Profilins
  • Proteins
  • profilactin
  • N-(1-pyrenyl)iodoacetamide
  • Cysteine
  • Ethylmaleimide
  • Iodoacetamide