Stimulatory proteins of RNA polymerase II from Ehrlich ascites tumor cells

Mol Cell Biochem. 1982 Aug 6;46(3):173-87. doi: 10.1007/BF00239666.

Abstract

Three proteins stimulating RNA polymerase II were purified from Ehrlich ascites tumor cells. Antibody was raised against one of these proteins. Immunofluorescent study revealed that these proteins ubiquitously exist in the nucleoplasm of various eukaryotic cells. This antibody specifically inhibited alpha-amanitin-sensitive RNA synthesis in isolated nuclei of Ehrlich ascites tumor cells and accurate transcription in HeLa cell lysate, indicating that these proteins are essential components of eukaryotic transcription. Two proteins were shown to have the same primary structure except that one of them is phosphorylated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinoma, Ehrlich Tumor / enzymology*
  • Cells, Cultured
  • DNA-Directed RNA Polymerases / metabolism*
  • Fluorescent Antibody Technique
  • Manganese / pharmacology
  • Mice
  • Molecular Weight
  • Neoplasm Proteins / isolation & purification*
  • Neoplasm Proteins / metabolism
  • Phosphorylation
  • RNA Polymerase II / metabolism*
  • Transcription Factors, General*
  • Transcriptional Elongation Factors*

Substances

  • Neoplasm Proteins
  • Transcription Factors, General
  • Transcriptional Elongation Factors
  • transcription factor S-II
  • Manganese
  • RNA Polymerase II
  • DNA-Directed RNA Polymerases