15N NMR study of a mixture of uniformly labeled tRNAs

Proc Natl Acad Sci U S A. 1982 Aug;79(16):4834-7. doi: 10.1073/pnas.79.16.4834.

Abstract

15N NMR spectra were taken of 15N-enriched tRNA extracted from bakers yeast; ammonium sulfate was used as a nitrogen source. The increase in the degree of denaturation of tRNA, which occurs with increase in temperature from 30 degrees C to 70 degrees C, resulted in no large changes in 15N chemical shifts at acidic and neutral pH but quite pronounced changes in proton-15N nuclear Overhauser effects.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Hydrogen Bonding
  • Magnetic Resonance Spectroscopy
  • Nucleic Acid Conformation
  • RNA, Fungal
  • RNA, Transfer*
  • Saccharomyces cerevisiae

Substances

  • RNA, Fungal
  • RNA, Transfer