Metabolism of Saccharomyces cerevisiae envelope mannoproteins

Arch Microbiol. 1982 Aug;132(2):144-8. doi: 10.1007/BF00508720.


By pulse and chase labeling experiments, two independent mannoprotein pools have been found associated with the Saccharomyces cerevisiae envelope. One of them probably corresponds to mannoproteins localized in the periplasmic space. These molecules showed a high turnover rate at 28 degrees C. The second pool is formed by intrinsic wall mannoproteins which are apparently stable for long periods of time, after a small initial turnover. These results suggest that at least part of the mannoproteins initially found in the periplasmic space may move into the wall. The time lag between the addition of the radioactive precursors and their incorporation in the cell envelope (20-30 min for amino acids and about 10 min for carbohydrate) indicates that protein formation and carbohydrate incorporation take place in succession. Moreover, bulk glycosylation of mannoproteins seems to occur close in time to the moment of secretion into the periplasmic space.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Wall / metabolism
  • Fungal Proteins / metabolism*
  • Glucan Endo-1,3-beta-D-Glucosidase / pharmacology
  • Glycoproteins / metabolism*
  • Kinetics
  • Mannans / metabolism
  • Membrane Glycoproteins*
  • Saccharomyces cerevisiae / metabolism*


  • Fungal Proteins
  • Glycoproteins
  • Mannans
  • Membrane Glycoproteins
  • mannoproteins
  • Glucan Endo-1,3-beta-D-Glucosidase