Circularization of linear viroid RNA via 2'-phosphomonoester, 3', 5'-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas

Nucleic Acids Res. 1982 Dec 11;10(23):7521-9. doi: 10.1093/nar/10.23.7521.

Abstract

A novel type of RNA ligase activity in extracts of wheat germ or Chlamydomonas requires 2', 3'-cyclic phosphate and 5'-phosphate ends for ligation to form a 2'-phosphomonoester, 3',5'-phosphodiester bond. Using 5'-3 2P-labeled linear PSTV, we demonstrate that RNase T1-nicked viroid predominantly forms (formula; see text) U-bonds. Natural linear PSTV, however, forms mainly (formula; see text) A-bonds upon enzymatic circularization. We show that natural linear PSTV RNA has nicks between C181 and A182, or between C348 and A349, and that consequently C181 and C348 carry 2',3'-cyclophosphate termini.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chemical Phenomena
  • Chemistry
  • Chlamydomonas / enzymology*
  • Phosphorus Radioisotopes
  • Plants / enzymology*
  • Polynucleotide Ligases / metabolism*
  • RNA Ligase (ATP) / metabolism*
  • RNA, Viral*
  • Radioisotope Dilution Technique
  • Triticum / enzymology
  • Viroids / analysis*

Substances

  • Phosphorus Radioisotopes
  • RNA, Viral
  • Polynucleotide Ligases
  • RNA Ligase (ATP)