Localisation of mini-Mu in its replication intermediates

EMBO J. 1982;1(8):965-9. doi: 10.1002/j.1460-2075.1982.tb01279.x.

Abstract

We have located Mu delta 26 sequences straddling the forks in DNA structures which appear during Mu delta 26 replication, i.e., keys, pending keys, dumb- bells , partially fused circles, and asymmetrical forks. This brings additional evidence that these structures are mini-Mu replication intermediates. The possible relationship between these structures and those predicted by the different models formulated to explain transposition in procaryotes is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coliphages / genetics*
  • DNA Replication*
  • DNA, Bacterial / genetics*
  • DNA, Viral / genetics*
  • Escherichia coli / genetics*
  • Microscopy, Electron
  • Nucleic Acid Denaturation
  • Virus Replication

Substances

  • DNA, Bacterial
  • DNA, Viral