Abstract
1. beta-D-Galactopyranosylmethyl-p-nitrophenyltriazene can specificag detectable changes in cell viability or the activities of other hydrolases. 2. beta-D-Glucopyranosylmethyl-p-nitrophenyltriazene behaves similarly towards lysosomal beta-glucosidase, but also inactivates some alpha-glucosidase. 3. Both beta-galactosidase and beta-glucosidase activities in triazene-treated confluent fibroblast cultures recover exponentially; if zero-order enzyme production is assumed, turnover times of 10 and 5 days respectively can be estimated.
MeSH terms
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Cells, Cultured
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Fibroblasts / drug effects*
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Fibroblasts / enzymology
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Galactosidases / antagonists & inhibitors*
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Galactosides / pharmacology*
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Glucosidases / antagonists & inhibitors*
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Glucosides / pharmacology*
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Glycosides / pharmacology*
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Humans
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Hydrolases / antagonists & inhibitors
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Lysosomes / drug effects
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Lysosomes / enzymology*
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Protein Denaturation / drug effects
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Triazenes / pharmacology*
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beta-Galactosidase / antagonists & inhibitors*
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beta-Glucosidase / antagonists & inhibitors*
Substances
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Galactosides
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Glucosides
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Glycosides
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Triazenes
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beta-D-galactopyranosylmethyl-4-nitrophenyltriazene
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beta-D-glucopyranosyl-4-nitrophenyltriazene
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Hydrolases
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Galactosidases
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Glucosidases
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beta-Glucosidase
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beta-Galactosidase