Amino-acid sequence of the L-1 light chain of chicken cardiac-muscle myosin

Eur J Biochem. 1980 Jun;107(2):565-75. doi: 10.1111/j.1432-1033.1980.tb06064.x.


The light chain fraction was separated from myosin extracted from chicken cardiac muscle. Two light chain components, L-1 and L-2 in the fraction were isolated by chromatography on a column of DEAE-cellulose (DE-52) in the presence of4 M urea. After performic acid oxidation, the L-1 chain was digested with trypsin and the resulting peptides were isolated. The amino acid sequences of the peptides were established. The ordering of these tryptic peptides in the L-1 chain was deduced from the amino acid compositions and the partial sequences of peptic peptides from S-carboxymethylated L-1 chain. Comparing the whole sequence of the L-1 chain thus established with that of alkali light chain of rabbit skeletal muscle myosin, 67 amino acid substitutions and two insertions were recognized.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chickens
  • Myocardium / analysis*
  • Myosins / analysis*
  • Pepsin A
  • Peptide Fragments / analysis
  • Trypsin


  • Peptide Fragments
  • Trypsin
  • Pepsin A
  • Myosins