Tk polyagglutination produced in vitro by an endo-beta-galactosidase

Vox Sang. 1980 Feb;38(2):94-8. doi: 10.1111/j.1423-0410.1980.tb02336.x.


After treatment with the culture supernatant of Bacteroides fragilis or with an endo-beta-galactosidase from Escherichia freundii, the human red blood cells are Tk-activated, i.e. agglutinable by BS II lectin and their blood group I and i activities are reduced. These results suggest that B. fragilis produces an endo-beta-galactosidase activity and that the main antigenic determinant of Tk polyagglutinable red cells is a terminal N-acetyl-glucosamine residue.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Agglutination / drug effects*
  • Bacteroides fragilis / enzymology
  • Bacteroides fragilis / immunology
  • Erythrocytes / drug effects
  • Erythrocytes / immunology
  • Erythrocytes / metabolism
  • Escherichia / enzymology
  • Galactosidases / pharmacology*
  • Glycoside Hydrolases*
  • beta-Galactosidase / metabolism
  • beta-Galactosidase / pharmacology*


  • Galactosidases
  • Glycoside Hydrolases
  • keratan-sulfate endo-1,4-beta-galactosidase
  • beta-Galactosidase