High-pressure liquid chromatographic determination of ftorafur [1-(tetrahydro-2-furanyl)-5-fluorouracil] and GLC-mass spectrometric determination of 5-fluorouracil and uracil in biological materials after oral administration of uracil plus ftorafur

J Pharm Sci. 1980 Nov;69(11):1296-300. doi: 10.1002/jps.2600691117.


Oral administration of uracil plus ftorafur [1-(tetrahydro-2-furanyl)-5-fluorouracil] has a greater antitumor effect than that of ftorafur alone. A high-pressure liquid chromatographic methods was developed for the determination of ftorafur in plasma and visceral tissues with a sensitivity of 0.025 microgram/ml or g of wet weight. A combination of GLC--mass fragmentography and GLC--mass spectrometry with total-ion monitoring was developed for the specific, simultaneous determination of 5-fluorouracil, and active metabolite, and uracil as their trimethylsilylated derivatives. The detection limits for 5-fluorouracil and uracil in the first method were 0.001 microgram/ml for plasma and 0.001--0.005 microgram/ml for visceral tissues, and those in the second method were 0.2 microgram/ml or g of wet weight. The precision and sensitivity of the assay appear to be satisfactory for determination of the levels of these compounds in plasma and visceral tissues.

MeSH terms

  • Administration, Oral
  • Animals
  • Chromatography, High Pressure Liquid*
  • Fluorouracil / analogs & derivatives*
  • Fluorouracil / analysis*
  • Gas Chromatography-Mass Spectrometry*
  • Male
  • Neoplasms, Experimental / drug therapy
  • Neoplasms, Experimental / metabolism
  • Rats
  • Tegafur / administration & dosage
  • Tegafur / analysis*
  • Uracil / administration & dosage
  • Uracil / analysis*


  • Tegafur
  • Uracil
  • Fluorouracil