Metabolism of [14C] amitraz in larvae of Boophilus microplus

Aust J Biol Sci. 1978 Apr;31(2):141-8. doi: 10.1071/bi9780141.

Abstract

Amitraz, 1, 5-di(2, 4-dimethylphenyl)-3-methyl-1, 3, 5-triazapenta-1, 4-diene, labelled with 14C in the 2-methyl groups was applied to B. microplus larvae by an immersion technique. The chemical penetrated readily but never appeared in large amounts internally due to rapid cleavage to N-2, 4-dimethylphenyl-N'-methylformamidine. The expected complementary cleavage product 2, 4-dimethylformanilide was not produced in equivalent quantity. However, large amounts of polar metabolite(s) were produced. Small quantities of 2, 4-dimethylaniline and an unidentified non-polar metabolite were also produced. Of the identified chemicals only amitraz and N-2, 4-dimethylphenyl-N'-methylformamidine were toxic to larvae. Piperonyl butoxide applied simultaneously with amitraz had only a slight effect on metabolism but had a three-fold synergistic effect. SKF 525-A similarly applied had a negligible effect on both metabolism and toxicity.

MeSH terms

  • Animals
  • Carbon Dioxide / metabolism
  • Drug Resistance
  • Drug Synergism
  • Insecticides / metabolism*
  • Larva / metabolism
  • Piperonyl Butoxide
  • Proadifen
  • Ticks / metabolism*
  • Toluidines / metabolism*

Substances

  • Insecticides
  • Toluidines
  • Carbon Dioxide
  • amitraz
  • Proadifen
  • Piperonyl Butoxide