The mucociliary activity of the respiratory tract. I. Inhibitory effects of products of Pseudomonas aeruginosa on rabbit trachea in vitro

Acta Otolaryngol. 1980 Nov-Dec;90(5-6):462-9. doi: 10.3109/00016488009131749.

Abstract

Bacteriological filtrates of three strains of Pseudomonas aeruginosa were compared with respect to inhibitory effect on ciliary movements and a quantitative difference was established between them. The cilia inhibitory effect was strictly concentration-dependent and was resistant to heating. The ciliotoxic effect disappeared from filtrates after chloroform extraction. The chloroform-extracted sediment ws dissolved in physiological saline and the solution revealed an inhibitory effect on cilia. Involvement of endotoxin is not probable, since E. coli endotoxin in a high concentration was not toxic. Partially purified Pseudomonas aeruginosa phenazine pigment as well as haemolysin inhibited ciliary activity and the effect was standardized in the present experimental system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteriological Techniques
  • Cilia / physiology*
  • Hemolysin Proteins / pharmacology
  • Mucus / physiology*
  • Pseudomonas aeruginosa / isolation & purification*
  • Pyocyanine / pharmacology
  • Rabbits
  • Trachea / physiology*

Substances

  • Hemolysin Proteins
  • Pyocyanine